Italian Journal of Mycology
https://italianmycology.unibo.it/
<strong>Italian Journal of Mycology – ISSN 2531-7342</strong> is a peer reviewed journal founded in 1972 by Gilberto Govi and Gabriele Goidanich as "Micologia italiana" (Italian Mycology). It was established at the University of Bologna, and it is organ of the “Unione Micologica Italiana”. Since 2015 it is an open access journal.DISTAL - Alma Mater Studiorum - Università di Bolognaen-USItalian Journal of Mycology2531-7342<p>Copyrights and publishing rights of all the texts on this journal belong to the respective authors without restrictions.</p><div><a href="http://creativecommons.org/licenses/by-nc/3.0/" rel="license"><img src="https://i.creativecommons.org/l/by/4.0/88x31.png" alt="Creative Commons License" /></a></div><p>This journal is licensed under a <a href="http://creativecommons.org/licenses/by/4.0/" rel="license">Creative Commons Attribution 4.0 International License</a> (<a href="http://creativecommons.org/licenses/by/4.0/legalcode">full legal code</a>). <br />See also our <a href="/about/editorialPolicies#openAccessPolicy">Open Access Policy</a>.</p>Three new species found in the Bracciano-Martignano Regional Natural Park in Lazio, Italy
https://italianmycology.unibo.it/article/view/18172
<p>We report the finding of three recently described species in late autumn and winter (October to December) in the Bracciano-Martignano Regional Natural Park in Lazio (Italy). The species are <em>Amanita calida</em>,<em> Lepiota elseae</em> and <em>Xerocomellus</em> <em>sarnarii</em>. This is the first record of <em>Lepiota elseae</em> for Italy. The collection site, a <em>Quercus </em>thicket, is located on the slopes of San Bernardino del Malpasso in Trevignano Romano, between Monte Rocca Romana (Sabatini mountains) and the nearby Bracciano Lake. A description of the specimens is given, and a comparison is made with the few descriptions of the ambient available for previous records of the respective species.</p>Amalia FerrettiArnold KnijnIrja Saar
Copyright (c) 2024 Amalia Ferretti, Arnold Knijn, Irja Saar
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2024-02-052024-02-055311210.6092/issn.2531-7342/18172Metabolic profiling of antimicrobial secondary metabolites produced by <em>Penicillium bilaiae</em> EWB-3 isolated from electronic wastes in Algeria
https://italianmycology.unibo.it/article/view/18345
<p><em>Penicillium</em> species research has progressed far beyond their ability to produce secondary metabolites with potential biological applications, particularly as antimicrobial agents. In this work, <em>Penicillium bilaiae</em> EWB-3 was isolated from electronic waste and identified using morphological and molecular (ITS and β-tubulin regions) methods. For 15 days, <em>Penicillium bilaiae</em> EWB-3 was grown into Czapek Yeast Broth using an orbital shaker. Finally, the secondary metabolites in this strain’s filtrates were extracted using ethyl acetate. The agar well diffusion method tested this crude extract for antimicrobial activity. The <em>Penicillium bilaiae</em> EWB-3 extract exhibited strong antimicrobial potential against all tested microorganisms, including <em>Pseudomonas aeruginosa, Bacillus cereus, Enterococcus faecalis, Escherichia coli, Salmonella typhimurium, Staphylococcus aureus</em>, Methicillin-resistant <em>Staphylococcus aureus, Klebsiella pneumoniae, Candida albicans</em> and <em>Aspergillus niger</em>. With diameters of 31, 26, and 25 mm, the largest inhibition zones were observed against <em>C. albicans, S. aureus,</em> and <em>E. faecalis,</em> respectively. The presence of 24 active compounds was revealed by gas chromatography-mass spectrometry (GC-MS) analysis of the crude extract of <em>Penicillium bilaiae</em> EWB-3. The findings suggest that the secondary metabolite extract of <em>Penicillium bilaiae</em> EWB-3 could be a promising active pharmaceutical component.</p>Dounya Achwak ChemmamGhania BourzamaNouari SadratiMoussa Houhamdi
Copyright (c) 2024 Dounya Achwak Chemmam, Ghania Bourzama, Nouari Sadrati, Moussa Houhamdi
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2024-02-052024-02-0553132810.6092/issn.2531-7342/18345Isolation, characterization and pathogenicity of fungal pathogens from indigenous postharvest fruits in Akwa Ibom state, Nigeria
https://italianmycology.unibo.it/article/view/16813
<p>The study investigated fungal pathogens associated with spoilage of five indigenous fruits <em>(Persia americana</em>, <em>Citrus sinensis</em>, <em>Carica papaya</em>, <em>Annona muricata</em> and <em>Solanum lycopersicum</em>) in Akwa Ibom State, Nigeria. Diseased fruit samples were purchased from nine markets located in three senatorial districts of the State. The phenotypic and genotypic identifications of the fungal pathogens isolated from spoilt fruit samples were carried out using standard cultural, morphological and molecular methods, respectively. The phylogenetic relationship among the fungal species was also constructed using neighbor-joining phylogenetic tree generated based on modified Rogers' genetic distance matrix. The fourteen fungal genera identified (and classified into twenty-two species) were <em>Talaromyces</em>, <em>Lasiodiplodia</em>, <em>Trichoderma</em>, <em>Penicillium</em>, <em>Pichia</em>, <em>Rhizopus</em>, <em>Aspergillus</em>, <em>Fusarium</em>, <em>Moniliella</em>, <em>Mucor</em>, <em>Geotrichum</em>, <em>Candida</em>, <em>Absidia</em> and <em>Purpureocillium</em>. Pathogenicity tests revealed that the fungal isolates were able to cause rots with a range of severity. The most rapid rots were caused by <em>Rhizopus oligosporous</em>, <em>Pichia kudriavzevii</em> and <em>Aspergillus niger</em> within 24 h, while <em>Aspergillus aculeatus</em> and <em>Moniliella suaveolens</em> were slower in initiating rots with earliest being after 48 h. The identification of fungi related with fruit rots and data coming from pathogenicity test are crucial information in order to plan and to apply control strategies during postharvest storage.</p>Ofon Mbuk Divine-AnthonyAnthony Ayodeji AdegokeOlusola T. OduoyeOghenerobor B. Akpor
Copyright (c) 2024 Ofon Mbuk Divine-Anthony, Anthony Ayodeji Adegoke, Olusola T. Oduoye, Oghenerobor B. Akpor
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2024-03-182024-03-1853294410.6092/issn.2531-7342/16813Overview of molecular methods for quick identification of <em>Tuber</em> species
https://italianmycology.unibo.it/article/view/19004
<p>Truffles represent a resource of great value all over the world. The various species are widespread and occupy various habitats. Methods to identify their value in terms of marketing, cultivation, and the ecological–forestal field are needed. The traditional classification of <em>Tuber</em> species is based on their morphology; nevertheless, the macroscopic and microscopic characteristics of the fruiting body, spores, and ectomycorrhizae may be absent or insufficiently discriminating. This problem is amplified when dealing with valuable truffle species, which are often replaced by truffles with lower organoleptic qualities and commercial value. Today, molecular biology can help to correctly identify them through several widely used methods that are fast, specific, and sensitive. Molecular methods are mostly PCR-based, with primers designed on various targets, genes, or regions widely variable between species but sufficiently conserved within the same species. The DNA regions analyzed for identifying truffles include ribosomal internal transcribed spacer (ITS), ribosomal large subunit (LSU), β-tubulin, and EF-α elongation factor. This review summarizes the main and most reliable DNA-based molecular methods for identifying <em>Tuber</em> species. The methods are listed schematically and clearly for quick consultation and use in all areas of study in which monitoring, and verification of the species is essential.</p>Antonella AmicucciBianca Ranocchi
Copyright (c) 2024 Antonella Amicucci, Bianca Ranocchi
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2024-03-182024-03-1853456410.6092/issn.2531-7342/19004