Coniella granati and Phytophthora palmivora the main pathogens involved in pomegranate dieback and mortality in north-eastern Italy
DOI:
https://doi.org/10.6092/issn.2531-7342/11170Keywords:
emerging diseases, Neofusicoccum, Botryosphaeria, Phytopythium, Cytospora, pathogenicityAbstract
Severe branch dieback, root rot and sudden death symptoms have recently been observed on pomegranate in several orchards in Veneto (north-eastern Italy). Since there is little information about the aetiology of these diseases and given the growing economic interest in this crop, an in-depth study was conducted. From winter 2017 to autumn 2018, forty-five symptomatic plants in two orchards were monitored and sampled to isolate the associated pathogens. Based on morphology, colony appearance and DNA sequence data, eight species belonging to seven genera and five families were isolated and identified. These included Botryosphaeria dothidea and Neofusicoccum parvum (Botryosphaeriaceae), Diaporthe eres (Diaporthaceae), Coniella granati (Schizoparmaceae), Cytospora punicae (Valsaceae), Phytophthrora palmivora, P. pseudocryptogea and Phytopythium vexans (Peronosporaceae). Pathogenicity trials conducted on 3-year-old pomegranate seedlings showed that three species, C. granati, N. parvum and P. palmivora are aggressive pathogens on pomegranate. Results obtained have allowed us to establish that the number of pathogens associated with emerging diseases of pomegranate is greater than previously recognised. Further studies are necessary to determine a possible synergistic interaction
References
Adiletta G, Petriccione M, Liguori L, Pizzolongo F, Romano R, Di Matteo M (2018) Study of pomological traits and physico-chemical quality of pomegranate (Punica granatum L.) genotypes grown in Italy. European Food Research Technology 244(8):1427–1438. https://doi.org/10.1007/s00217-018-3056-x
Basu A, Penugonda K (2009) Pomegranate juice: a heart healthy fruit juice. Nutrition Reviews 67:49–56. https://doi.org/10.1111/j.1753-4887.2008.00133.x
Borines LM, Palermo VG, Guadalquiver GA, Dwyer C, Drenth A, Daniel R, Guest DI (2014) Jackfruit decline caused by Phytophthora palmivora (Butler). Australasian Plant Pathology 43:123–129. https://doi.org/10.1007/s13313-013-0241-z
Calani L, Beghè D, Mena P, Del Rio D, Bruni R, Fabbri A, Dall’Asta C, Galaverna G (2013) Ultra-HPLC-MSn (poly)phenolic profiling and chemometric analysis of juices from ancient Punica granatum L. cultivars: a non targeted approach. Journal Agriculture Food Chemistry 61:5600–5609. https://doi.org/10.1021/jf400387c
Çeliker NM, Uysal A, Çetinel B, Poyraz D, (2012) Crown rot on pomegranate caused by Coniella granati in Turkey. Australasian Plant Disease Notes 7:161–162. https://doi.org/10.1007/s13314-012-0074-6
Chen Y, Shao DD, Zhang AF, Yang X, (2014) First report of a fruit rot and twig blight on pomegranate (Punica granatum) caused by Pilidiella granati in Anhui province of China. Plant Disease 89:695. https://doi.org/10.1094/PDIS-09-13-1012-PDN
Derevnina L, Petre B, Kellner R, Dagdas YF, Sarowar MN, Giannakopoulou A, De la Concepcion, JC, Chaparro-Garcia A, Pennington HG, van West P, Kamoun S (2016) Emerging oomycete threats to plants and animals. Philosophical Transactions of the Royal Society B 371: 20150459. https://doi.org/10.1098/rstb.2015.0459
Erwin CD, Ribeiro OK (1996) Phytophthora Diseases Worldwide. St. Paul, MN: American Phytopathological Society Press, 562 pp.
Ferrara G, Cavoski I, Pacifico A, Tedone L, Mondelli D (2011) Morpho-pomological and chemical characterization of pomegranate (Punica granatum L.) genotypes in Apulia region, Southeastern Italy. Scientia Horticulturae 130:599–606. https://doi.org/10.1016/j.scienta.2011.08.016
Ferrara G, Giancaspro A, Mazzeo A, Giove SL, Matarrese AMS, Pacucci C, Punzi R, Trani A, Gambacorta G, Blanco A, Gadaleta A (2014) Characterization of pomegranate (Punica granatum L.) genotypes collected in Puglia region, Southeastern Italy. Scientia Horticulturae 178:70–78. https://doi.org/10.1016/j.scienta.2014.08.007
Guest DI (2007) Black pod: Diverse pathogens with a global impact on cocoa yield. Phytopathology 97:1650–1653. https://doi.org/10.1094/PHYTO-97-12-1650
Holland D, Hatib K, Bar-Yàakov I (2008) Pomegranate: botany, horticulture, breeding. Horticultural Reviews 35:127–191. https://doi.org/10.1002/9780470593776.ch2
Jabnoun-Khiareddine H, Ibrahim N, Aydi Ben Abdallah R, Mars M, Kthiri Z, Daami-Remadi M (2018) Coniella granati (Saccardo) a new potential threat to pomegranate (Punica granatum L.) in Tunisia causing twig dieback and Fruit Rot. Journal Plant Pathology & Microbiology 9:450. https://doi.org/10.4172/2157-7471.100045
KC A, Vallad G (2016) First report of Neofusicoccum parvum causing shoot blight and stem cankers on pomegranate in Florida. Plant Disease 100:1783. https://doi.org/10.1094/PDIS-01-16-0067-PDN
Kurbetli İ, Karaca G, Aydoğdu M, Sülü G (2020) Phytophthora species causing root and collar rot of pomegranate in Turkey. European Journal of Plant Pathology 157:485–496. https://doi.org/10.1007/s10658-020-02007-8
Lansky PE, Newman RA (2007) Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology 109:177–206. https://doi.org/10.1016/j.jep.2006.09.006
Lennox CL, Mostert L, Venter E, Laubscher W, Meitz-Hopkins JC (2018) First report of Coniella granati fruit rot and dieback on pomegranate in the western cape of South Africa. Plant Disease 102:821. https://doi.org/10.1094/PDIS-09-17-1387-PDN
Linaldeddu BT, Scanu B, Maddau L, Franceschini A (2014) Diplodia corticola and Phytophthora cinnamomi: the main pathogens involved in holm oak decline on Caprera Island (Italy). Forest Pathology 44:191–200. https://doi.org/10.1111/efp.12081
Linaldeddu BT, Alves A, Phillips AJL (2016) Sardiniella urbana gen. et sp. nov., a new member of the Botryosphaeriaceae isolated from declining Celtis australis trees in Sardinian streetscapes. Mycosphere 7:893–905. https://doi.org/10.5943/mycosphere/si/1b/5
Linaldeddu BT, Bregant C, Montecchio L, Favaron F, Sella L (2019) Characterization of Phytophthora and Botryosphaeriaceae species associated with emerging olive diseases in northeast Italy. Journal of Plant Pathology 101(4):829. https://doi.org/10.1007/s42161-019-00394-4
Linaldeddu BT, Mulas AA, Bregant C, Piras G, Montecchio L (2020) First report of Phytophthora pistaciae causing root and collar rot on nursery plants of Pistacia lentiscus in Italy. Plant Disease 104(5):1564. https://doi.org/10.1094/PDIS-12-19-2567-PDN
Maloney PE, Lynch SC, Kane SF, Rizzo DM (2004) Disease progression of Phytophthora ramorum and Botryosphaeria dothidea on pacific madrone. Plant Disease 88:852–857. https://doi.org/10.1094/PDIS.2004.88.8.852
Markakis EA, Tzima AK, Palavouzis SC, Antoniou PP, Paplomatas EJ, Tjamos EC (2017) First report of Phytophthora palmivora causing fruit rot on pomegranate in Greece. Plant Disease 101:1060. https://doi.org/10.1094/PDIS-11-16-1691-PDN
Mars M (2000) Pomegranate plant material genetic resources and breeding, a review. Options Mediterranéenees, Série A 42:55–62. http://om.ciheam.org/om/pdf/a42/00600252.pdf
Michailides TJ, Pucket R, Morgan D (2010) Pomegranate decay caused by Pilidiella granati in California. Phytopathology 100:S83.
Mirabolfathy M, Groenewald JZ, Crous PW (2012) First report of Pilidiella granati causing dieback and fruit rot of pomegranate (Punica granatum) in Iran. Plant Disease 96:461. https://doi.org/10.1094/PDIS-10-11-0887
Palou L, Guardado A, Montesinos-Herrero C (2010) First report of Penicillium spp. and Pilidiella granati causing postharvest fruit rot of pomegranate in Spain. New Disease Reports 22:21. http://dx.doi.org/10.5197/j.2044-0588.2010.022.021
Peduto Hand F, Choudhury RA, Gubler WD (2014) First report of Cytospora punicae causing wood canker and branch dieback of pomegranate (Punica granatum) in the United States. Plant Disease 98:853. https://doi.org/10.1094/PDIS-11-13-1133-PDN
Peter PK, Chandramohan R (2014) Integrated management of black pod disease of cocoa caused by Phytophthora palmivora. International Journal of Plant Protection 7(1):107–110.
Pollastro S, Dongiovanni C, Gerin D, Pollastro P, Fumarola G, De Miccolis A, Faretra F (2016) First report of Coniella granati (Sacc.) Petr. & Syd. as a causal agent of pomegranate crown rot in Southern Italy. Plant Disease 100(7):1498. https://doi.org/10.1094/PDIS-11-15-1320-PDN
Riccioni L, Valente MT, Di Gianbattista G (2017) First report of Neofusicoccum parvum causing shoot blight and plant decay on pomegranate in Tarquinia, Italy. Journal Plant Pathology 99(1):294. http://dx.doi.org/10.4454/jpp.v99i1.3805
Safaiefarahani B, Mostowfizadeh-Ghalamfarsa R, Hardy GESJ, Burgess TI (2015) Re-evaluation of the Phytophthora cryptogea species complex and the description of a new species, Phytophthora pseudocryptogea sp. nov. Mycological Progress 14:1–12. https://doi.org/10.1007/s11557-015-1129-9
Seeram NP, Aviram M, Zhang Y, Henning S, Feng L, Dreher M, Heber D (2008) Comparison of antioxidant potency of commonly consumed polyphenol-rich beverages in the United States. Journal Agriculture Food Chemistry 56:1415–1422. https://doi.org/10.1021/jf073035s
Smahi H, Belhoucine-Guezouli L, Berraf-Tebbal A, Chouih S, Arkam M, Franceschini A, Linaldeddu BT, Phillips AJL (2017) Molecular characterization and pathogenicity of Diplodia corticola and other Botryosphaeriaceae species associated with canker and dieback of Quercus suber in Algeria. Mycosphere 8(2):1261–1272. https://doi.org/10.5943/mycosphere/8/2/10
Thomidis T (2015) Pathogenicity and characterization of Pilidiella granati causing pomegranate diseases in Greece. European Journal of Plant Pathology 141:45–50. https://doi.org/10.1007/s10658-014-0520-8
Thomidis T, Exadaktylou E (2011) First Report of Pilidiella granati on pomegranate with symptoms of crown rot in the prefecture of Xanthi, Greece. Plant Disease 95(1):79. https://doi.org/10.1094/PDIS-07-10-0514
Torres GA, Sarria GA, Martinez G, Varon A, Drenth A, Guest DI (2016) Bud rot caused by Phytophthora palmivora: a destructive emerging disease of oil palm. Phytopathology 106:320–329. https://doi.org/10.1094/PHYTO-09-15-0243-RVW
Triki MA, Gharbi Y, Cheffi M, Krid SR, Hammami I, Hamdi H, Chaabani B, Ali HB, Gdoura R (2015) First report of Cytospora punicae associated with wood canker and branch dieback disease of pomegranate in Tunisia. Journal Plant Pathology 97:541–551. http://dx.doi.org/10.4454/JPP.V97I3.039
Uysal A, Kurt Ş (2018) Colletotrichum gloeosporiodes causing anthracnose on pomegranate in Turkey. Australasian Plant Disease Notes 13:19. https://doi.org/10.1007/s13314-018-0303-8
White TJ, Bruns T, Lee J, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds), PCR protocols: a guide to methods and applications. Academic Press, San Diego, California, USA, pp 315–322.
Xavier KV, Nepal Kc A, Crous PW, Groenewald JZ, Vallad GE (2019) Dwiroopa punicae sp. nov. (Dwiroopaceae fam. nov., Diaporthales), associated with leaf spot and fruit rot of pomegranate (Punica granatum). Fungal Systematics and Evolution 4:33–41. https://doi.org/10.3114/fuse.2019.04.04
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