Rare findings from the chestnut forest of Monte Rocca Romana (Latium, Italy)





Hydnum ibericum, Hydnum ovoideisporum, Entoloma, Cyanula, Ramariopsis pulchella, chestnut forest, Natural Parks


We report the identification of both Hydnum ibericum and H. ovoideisporum growing in late autumn, in the woods of Monte Rocca Romana, the highest peak (612 m) of Monti Sabatini, inside the Bracciano-Martignano Regional Natural Park in Latium, Italy. The Park includes large composite zones: the relatively humid climate and the fertile volcanic soils allow the growth of a manifold flora related to the Mediterranean scrub, to the deciduous (Quercus spp.) or chestnut (Castanea sativa) and depressed beech woods. The fungal findings were observed in an area of the Park characterised by a forest composed in prevalence of chestnut trees and underwood of Ilex aquifolium and Ruscus aculeatus. In the same habitat and period, two small fungal species were present: blue coloured Entoloma sp. and Ramariopsis pulchella. The Entoloma sp. belongs to the Cyanula subgenus but combined morphological and molecular criteria did not correspond to any described Entoloma species yet. Ramariopis pulchella is a rare and ecologically important species.


Agerer R, Kraigher H, Javornik B (1996) Identification of ectomycorrhizae of Hydnum rufescens on Norway spruce and the variability if the ITS region of H. rufescens and H. repandum (Basidiomycetes). Nova Hedwigia 63:183-194.

Arnolds E (1982) Ecology and coenology of macrofungi in grasslands and moist heathlands in Drenthe, the Netherlands. Part I. Introduction and Synecology. Bibliotheca Mycologica. 90:341-343.

Arnolds E, Kuyper ThW, Noordeloos EM (eds) (1995) Overzicht van de paddestoelen in Nederland. Nederlandse Mycologische Vereniging, Wijster.

Baroni TJ, Hofstetter V, Largent DL, Vilgalys R (2011) Entocybe is proposed as a new genus in the Entolomataceae (Agaricomycetes, Basidiomycota) based on morphological and molecular evidence. North America Fungi, 6: 1-19. https://doi.org/10.2509/naf2011.006.012

Boudier É (1887) Clavaria pulchella Boud. Bulletin de la Société Mycologique de France 3(2):146.

Buyck B, Duhen B, Das K, Jayawardena RS, Niveiro N, Pereira OL, Prasher IB, Adhikari S, Alberto’ EO, Bulgakow TS, Castaneda-Ruiz RF, Hembrom ME, Hyde KD, Lewis DP, Michlig A, Nuithinck L, Parihar A, Popoff OF, Ramirez NA, Silva MD, Verma RK, Hofstetter V (2017) Fungi biodiversity profiles 21-30. Cryptogamie Mycologie 38: 101-146. https://doi.org/10.7872/crym/v38.iss1.2017.101

Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL (2009) BLAST+: architecture and applications. BMC Bioinformatics 10:421-429. https://doi.org/10.1186/1471-2105-10-421

Cerulli A (2019) Aspetti storico amministrativi, ambientali e gestionali forestali. La Faggeta di Monte Raschio, 1-18. Parco naturale regionale di Bracciano-Martignano.

Clericuzio M, Vizzini A (2011) Towards a better knowledge of the Tuscan mycological flora. Rare or interesting Basidiomycota from the Province of Grosseto. I. Micologia e Vegetazione Mediterranea, 26:37-60.

Corner EJH (1950) A Monograph of Clavaria and Allied Genera. Annals of Botany Memoirs 1:645.

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32:1792-1797. https://doi.org/10.1093/nar/gkh340

Feng B, Wang XH, Ratkowsky D, Gates G, See Lee S, Grebenc T, Yang ZL (2016) Multilocus phylogenetic analyses reveal unexpected abundant diversity and significant disjunct distribution pattern of the Hedgehog Mushrooms (Hydnum L.). Scientific Reports 6:25586. https://doi.org/10.1038/srep25586

Ferretti A, Saar I, Knijn A (2020) On two endangered species (Myriostoma coliforme and Battarrea sp.) sharing the same microhabitat. Italian Journal of Mycology. 49:1-7. https://doi.org/10.6092/issn.2531-7342/10039

Grebenc T, Martín MP, Kraigher H (2009) Ribosomal ITS diversity among the European species of the genus Hydnum (Hydnaceae). Anales del Jardín Botánico de Madrid. 66(S1): 121-132. https://doi.org/10.3989/ajbm.2221

Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59:307-321. https://doi.org/10.1093/sysbio/syq010

He XL, Horak E., Li TH, Peng WH, Gan BC (2020) Corrigendum: He XL, Horak E, Wang D, Li TH, Peng WH, Gan BC (2019) Descriptions of five new species in Entoloma subgenus Claudopus from China, with molecular phylogeny of Entoloma s.l. MycoKeys 61: 1–26. MycoKeys 2020, 63:163-172. https://doi.org/10.3897/mycokeys.61.46446

Jindrich O (2012) Ramariopsis pulchella – a rare species of Czech nature. Mycologicky Sbornik 89:8-10.

Kõljalg U, Nilsson RH, Abarenkov K, Tedersoo L, Taylor AFS, Bahram M, Bates ST, Bruns TD, Bengtsson-Palme J, Callaghan TM, Douglas B, Drenkhan T, Eberhardt U, Dueñas M, Grebenc T, Griffith GW, Hartmann M, Kirk PM, Kohout P, Larsson E, Lindahl BD, Lücking R, Martín MP, Matheny PB, Nguyen NH, Niskanen T, Oja J, Peay KG, Peintner U, Peterson M, Põldmaa K, Saag L, Saar I, Schüßler A, Scott JA, Senés C, Smith ME, Suija A, Taylor DL, Telleria MT, Weiß M, Larsson KH (2013) Towards a unified paradigm for sequence-based identification of Fungi. Molecular Ecology 22:5271-5277. https://doi.org/10.1111/mec.12481

Larsson A (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30:3276-3278. https://doi.org/10.1093/bioinformatics/btu531

Lorås J, Weholt Ø, Eidissen SE (2014) Entoloma gomerense Wölfel & Noordel. – a new species to Northern Europe. Agarica 35:19-23.

Magrini S, Azzella MM, Bolpagni R, Zucconi L (2020) In vitro propagation of Isoëtes sabatina (Isoetaceae): A key conservation challenge for a critically endangered Quillwort. Plants 9: 887-902. https://doi.org/10.3390/plants9070887

Morozova O, Vila J (2014) Entoloma subgenus Leptonia in boreal-temperate Eurasia: Towards a phylogenetic species concept. Persoonia – Molecular Phylogeny and Evolution of Fungi. 32:141-169. https://doi.org/10.3767/003158514x681774

Niskanen T, Liimatainen K, Nuytinck J, Kirk P, Olariaga Ibargururen I, Garibay-Orijel R, Novell L, Huhtinen S, Kytovuori I, Ruotsalainen J, Niemela T, Ammirati JF, Tedersoo L (2018) Identifying and naming the currently known diversity of the genus Hydnum with an emphasis on European and North American taxa. Mycologia 110:890-918. https://doi.org/10.1080/00275514.2018.1477004

Noordeloos ME (1982) Entoloma subgenus Leptonia in Northwestern Europa—I. Introduction and a revision of its section Leptonia. Persoonia, 11:451-471.

Noordeloos ME, Lorås J, Eidissen SE, Brandrud Tor E, Bendiksen E, Morozova O, Jordal J B, Weholt Ø, Jansen GM, Larsson E, Dima B (2021) Three new Entoloma species of the Cyanula clade from (sub)alpine habitats in Northern Norway and Sweden. Sidowia 73:185-196. https://doi.org/10.12905/0380.sydowia73-2020-0185

Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University. https://github.com/nylander/MrModeltest2

Olariaga I, Grebenc T, Salcedo I, Martín MP (2012) Two new species of Hydnum with ovoid basidiospores: H. ovodeisporum and H. vesterholtii. Mycologia 104:1443-1455. https://doi.org/10.3852/11-378

Onofri S, Bernicchia A, Filipello Marchisio V, Padovan F, Perini C, Ripa C, Salerni E, Savino E, Venturella G, Vizzini A, Zotti M, Zucconi L (2005) Checklist of Italian fungi: Basidiomycota. Carlo Delfino, Florence.

Ostrow H, Beenken L (2004) Hydnum ellipsosporum spec. nov. (Basidiomycetes, Cantharellales) – ein Doppelganger von Hydnum rufescens Fr. Zeitschrift für Mykologie 70:137-156.

Ricci G, Perini C (2001) Ritrovamenti interessanti: Ramariopsis pulchella (Boud.) Corner. Rivista di Micologia 2:135–142.

Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539-542. https://doi.org/10.1093/sysbio/sys029

Salerni E, Perini C (2007) Mycodiversity of nature reserves in Central Italy. Acta Mycologica. 42:5-19.

Senn-Irlet B (2005) The role of the ECCF in studies and conservation of fungi in Europe. Mycologia Balcanica 2:185-192. https://doi.org/10.5281/zenodo.2547027

Senn-Irlet B, Bieri G, Egli S (2007) Lista Rossa delle specie minacciate in Svizzera: macromiceti. Serie Pratica ambientale n. 0718. Editore Ufficio federale dell’ambiente, Berna e WSL, Birmensdorf.

Shiryaev A (2009) Diversity and distribution of clavarioid fungi in Estonia. Folia Cryptogamica Estonica 45:65–80. https://ojs.utlib.ee/index.php/FCE/article/view/13674

Swenie RA, Baroni TJ, Matheny PB (2018) Six new species and reports of Hydnum (Cantharellales) from eastern North America. MycoKeys 42:35-72. https://doi.org/10.3897/mycokeys.42.27369

Various authors (2009) Proposta di Piano di Gestione e delle Misure di Conservazione per la Gestione della ZPS IT6030085 “Bracciano-Martignano” e SIC annessi. (Parco Nazionale Regionale ‘Bracciano-Martignano’ and Associazione HydranGea, eds).

UFAM (2011) Lista delle specie prioritarie a livello nazionale. Specie prioritarie per la conservazione e la promozione a livello nazionale, stato 2010. Ufficio federale dell’ambiente, Berna. Pratica ambientale n. 1103.

Vila J, Carbó J, Caballero F, Català S, Llimona X, Noordeloos M (2013) A first approach to the study of the genus Entoloma subgenus Nolanea s.l. using molecular and morphological data. Fungi non Delineati. 66 (Studies on Entoloma): 3-62.

Vizzini A, Picillo B, Ercole E, Voyron S, Contu M (2013) Detecting the variability of Hydnum ovoideisporum (Agaricomycetes, Cantharellales) on the basis of Italian collections, and H. magnorufescens sp. nov. Mycosphere 4:32-44. https://doi.org/10.5943/mycosphere/4/1/2

Voitk A, Saar I, Trudell S, Spirin V, Beug M, Kõljalg U (2018) Polyozellus multiplex (Thelephorales) is a species complex containing four new species. Mycologia 109:975-992. https://doi.org/10.1080/00275514.2017.1416246

Volobuev S, Bolshakov S (2017) Host preferences and distributional patterns of rare and protected species of aphyllophoroid fungi in the Middle Russian Upland. In: Proceedings of the conference Fungal conservation in a changing Europe (International Society for Fungal Conservation Ss. Cyril and Methodius University, Skopje, Macedonian Mycological Society, ed), 1-6 October 2017, Ohrid, Republic of Macedonia, p 61.




How to Cite

Knijn, A., Ferretti, A., & Saar, I. (2021). Rare findings from the chestnut forest of Monte Rocca Romana (Latium, Italy). Italian Journal of Mycology, 50, 78–91. https://doi.org/10.6092/issn.2531-7342/12562