A new species of Agaricus (section Sanguinolenti) from Rome, Italy





Pseudochitonia, single-nucleotide variants, urban park, Villa Borghese


We report the finding of an Agaricus specimen in the section Sanguinolenti and describe it as a new species Agaricus hortusdamarum. Based on ITS data, the species is distinct and well separated from all known sequenced species within this section. The type specimen grew in late autumn, under Cupressus sempervirens, near a Pinus pinea alley in the Villa Borghese Gardens in Rome, in Italy. The new species is phylogenetically close to Agaricus iranicus, a rare species of temperate climate, which is so far described only in the Guilan province in Iran. Its morphological characters and phylogenetic pattern are described and divergence from A. iranicus is discussed.


AMINT (2015) Tutto Funghi Cercarli, riconoscerli, raccoglierli. G. De Vecchi, Florence.

Basso MT (2012) Manuale di Microscopia dei Funghi. Vol 2: 8–13. Eds Libreria Mykoflora. Villanova D’Albegna.

Boekelheide K, Graham DG, Mize PD, Anderson CW, Jeffs PW (1979) Synthesis of gamma-L-[3,5-3H] 4-hydroxybenzene and the study of reaction catalysed by tyrosinase of Agaricus bisporus. Journal of Biological Chemistry 254:12185–12191. https://doi.org/10.1016/S0021-9258(19)86447-5

Bunyard BA, Nicholson MS, Royse DJ (1996) Phylogeny of the genus Agaricus inferred from restriction analysis of enzymatically amplified ribosomal DNA. Fungal Genetics and Biology 20:243–253. https://doi.org/10.1006/fgbi.1996.0039

Burton KS (1986) Quality-investigations into mushroom browning. The Mushroom Journal 158:68–70.

Callac P, Chen J (2018) Tropical species of Agaricus. In: Updates on Tropical Mushrooms. Basic and Applies Research (Sanchez JE, Mata G, Royse DJ, eds). Ecosur, San Cristobal de Las casas, Chiapas, pp 25-38.

Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden TL (2009) BLAST+: architecture and applications. BMC Bioinformatics 10:421–429. https://doi.org/10.1186/1471-2105-10-421

Campitelli A (2005) Green delights. The villas, gardens and historic parks belonging to the City of Rome. De Luca editori d’Arte.

Chen J, Zhao R-L, Parra LA, Guelly AK, De Kesel A, Rapior S, Hyde KD, Chukeatirote E, Callac P (2015) Agaricus section Brunneopicti: a phylogenetic reconstruction with descriptions of four new taxa. Phytotaxa 192:145–168. https://doi.org/10.11646/phytotaxa.192.3.2

Chen J, Callac P, Parra LA, Karunarathna SC, He M-Q, Moinard M, De kesel A, Raspé O, Wisitrassameewong K, Hyde KD, Zhao R-L (2017) Study in Agaricus subgenus Minores and allied clades reveals a new American subgenus and contrasting phylogenetic patterns in Europe and Greater Mekong Subregion. Persoonia 38:170–196. https://doi.org/10.3767/003158517X695521

Edgar RC (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32:1792-1797. https://doi.org/10.1093/nar/gkh340

Geml J, Geiser DM, Royse DJ (2004) Molecular evolution of Agaricus species based on ITS and LSU rDNA sequences. Mycological Progress 3:157–176. https://doi.org/10.1007/s11557-006-0086-8

Ghobad-Nejhad M, Langer E, Antonín V, Gates G, Noroozi J, Zare R (2020) The gilled fungi and boletes of Iran: diversity, systematics, and nrDNA data. Mycologia Iranica 1013:1–43.

Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59:307–321. https://doi.org/10.1093/sysbio/syq010

He MQ, Chuankid B, Hyde KD, Cheewangkoon R, Zhao RL (2018) A new section and species of Agaricus subgenus Pseudochitonia from Thailand. MycoKeys 40:53–67. https://doi.org/10.3897/mycokeys.40.26918

Jaichaliaw C, Kumla J, Vadthanarat S, Suwannarach N, Lumyong S (2021) Multigene phylogeny and morphology reveal three novel species and a novel record of Agaricus from northern Thailand. Frontiers in Microbiology 12:1–14. https://doi.org/10.3389/fmicb.2021.650513

Jolivet S, Voiland A, Pellon G, Arpin N (1995) Main factors involved in the browning of Agaricus bisporus. Mushroom Science 14:695–702.

Jolivet S, Mooibroek H, Wichers HJ (1998) Space-time distribution of γ-glutamyl transferase activity in Agaricus bisporus. FEMS Microbiology Letters 163:263–267. https://doi.org/10.1111/j.1574-6968.1998.tb13055.x

Kerrigan RW, Callac P, Guinberteau J, Challen MP, Parra LA (2005) Agaricus section Xanthodermatei: a phylogenetic reconstruction with commentary on taxa. Mycologia 97:1292–1315. https://doi.org/10.3852/mycologia.97.6.1292

Knijn A, Ferretti A (2018) Punctularia atropurpurascens in the Villa Ada urban Park in Rome, Italy. Italian Journal of Mycology 47:29–39. https://doi.org/10.6092/issn.2531-7342/8349

Kõljalg U, Nilsson RH, Abarenkov K, Tedersoo L, Taylor AFS, Bahram M, Bates ST, Bruns TD, Bengtsson-Palme J, Callaghan TM, Douglas B, Drenkhan T, Eberhardt U, Dueñas M, Grebenc T, Griffith GW, Hartmann M, Kirk PM, Kohout P, Larsson E, Lindahl BD, Lücking R, Martín MP, Matheny PB, Nguyen NH, Niskanen T, Oja J, Peay KG, Peintner U, Peterson M, Põldmaa K, Saag L, Saar I, Schüßler A, Scott JA, Senés C, Smith ME, Suija A, Taylor DL, Telleria MT, Weiß M, Larsson KH (2013) Towards a unified paradigm for sequence-based identification of fungi. Molecular Ecology 22:5271–5277. https://doi.org/10.1111/mec.12481

La Chiusa L (2013) Funghi Agaricoidi. Vol. 1 – Agaricaceae. ANDER Edizioni, Monza.

Larsson A (2014) AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics 30:3276–3278. https://doi.org/10.1093/bioinformatics/btu531

Mahdizadeh V, Safaie N, Goltapeh E, Asef MR, Hosseini S, Callac P (2016) Agaricus section Xanthodermatei in Iran. Phytotaxa 247:181–196. https://doi.org/10.11646/phytotaxa.247.3.2

Mahdizadeh V, Parra LA, Safaie N, Goltapeh EM, Chen J, Guinberteau J, Callac P (2018) A phylogenetic and morphological overview of sections Bohusia, Sanguinolenti, and allied sections within Agaricus subg. Pseudochitonia with three new species from France, Iran and Portugal. Fungal Biology 122:34–51. https://doi.org/10.1016/j.funbio.2017.10.001

Naveen C, Beelman RB (2006) Microbial spoilage of fresh mushrooms. In: Microbiology of Fruits and Vegetables (Sapers GM, Gorny JR, Yousef AE, eds). CRC Press Inc., Boca Raton, USA, pp 135–158.

O’Toole A, Tomkins-Tinch C (2021) https://github.com/aineniamh/snipit (accessed 25 April 2021).

Parra LA, Wisman J, Guinberteau J, Wilhelm M, Musumeci E, Callac P, Geml J (2015) Agaricus collegarum and Agaricus masoalensis, two new taxa of the section Nigrobrunnescentes collected in Europe. Micologia e Vegetazione Mediterranea 30:3–26.

Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61:539–542. https://doi.org/10.1093/sysbio/sys029

Soler-Rivas C, Arpin, N, Olivier JM, Wichers HJ (2000) Discoloration and tyrosinase activity in Agaricus bisporus fruit bodies infected by various pathogens. Mycological Research 104:351–356. https://doi.org/10.1017/S0953756299001343

Voitk A, Saar I, Trudell S, Spirin V, Beug M, Kõljalg U (2018) Polyozellus multiplex (Thelephorales) is a species complex containing four new species. Mycologia 109:975–992. https://doi.org/10.1080/00275514.2017.1416246

Wang ZR, Parra LA, Callac P, Zhou JL, Fu WJ, Dui SH, Hyde KD, Zhao RL (2015) Edible species of Agaricus (Agaricaceae) from Xinjiang Province (Western China). Phytotaxa 202:185–197. https://doi.org/10.11646/phytotaxa.202.3.2

Weijn A, van den Berg-Somhorst DBPM, Slootweg JC, Vincken J-P, Gruppen H, Wichers HJ, Mes JJ (2013) Main phenolic compounds of the melanin biosynthesis pathway in bruising-tolerant and bruising-sensitive button mushroom (Agaricus bisporus) strains. Journal of Agricultural and Food Chemistry 61:8224–8231. https://doi.org/10.1021/jf4020558

Zhao R, Karunarathna S, Raspé O, Parra LA, Guinberteau J, Moinard M, De Kesel A, Barroso G, Courtecuisse R, Hyde KD, Guelly AK, Desjardin DE, Callac P (2011) Major clades in tropical Agaricus. Fungal Diversity 51:279–296. https://doi.org/10.1007/s13225-011-0136-7

Zhao R-L, Zhou J-L, Chen J, Margaritescu S, Sánchez-Ramírez S, Hyde KD, Callac P, Parra LA, Li G-J, Moncalvo J-M (2016) Towards standardizing taxonomic ranks using divergence times – a case study for reconstruction of the Agaricus taxonomic system. Fungal Diversity 78:239–292. https://doi.org/10.1007/s13225-016-0357-x



2022-02-21 — Updated on 2024-01-22


How to Cite

Ferretti, A., Saar, I., & Knijn, A. (2022). A new species of Agaricus (section Sanguinolenti) from Rome, Italy. Italian Journal of Mycology, 51(1), 1–10. https://doi.org/10.6092/issn.2531-7342/13464