Screening and virulence of Iranian isolates of Beauveria bassiana for potential management of Sunn Pest (Eurygaster integriceps Puton) in relation to cold activity
DOI:
https://doi.org/10.6092/issn.2531-7342/15270Keywords:
Beauveria bassiana, cold activity, sunn pest, Eurygaster integriceps, IranAbstract
Environmental abiotic factors, such as low temperatures, restrict the application of entomopathogenic fungi well-known biological control agents against agricultural pests. Among 60 Iranian isolates collected in Central Iran that were exposed to cold stress, four isolates were identified as potential candidates for further investigation. One hundred percent of the conidia of these four isolates germinated and subsequently produced conidia. Their virulence against Sunn Pest (Eurygaster integriceps Puton) varied between isolates. After applying cold stress (4 °C), Vesh 1-8 isolate with sporulation of 6.5 × 10 7 conidia mL-1, 50% mortality and LT in 3/4 days was the most cold-tolerant isolate. Cold-active germination and growth of the biocontrol fungal isolates are important to industrialize the products with a high potential against target pests in different environmental conditions.
References
Borisade OA, Magan N (2014) Growth and sporulation of entomopathogenic Beauveria bassiana, Metarhizium anisopliae, Isaria farinosa and Isaria fumosorosea strains in relation to water activity and temperature interactions. Biocontrol Science and Technology 24(9):999‒1011. https://doi.org/10.1080/09583157.2014.909007
Davari A, Skinner M, Parker BL (2018) Cell electrofusion to improve efficacy and thermotolerance of the entomopathogenic fungus, Beauveria bassiana. Journal of applied microbiology 125(5):1482‒1493. https://doi.org/10.1111/jam.14031
Edgington S, Moore D, El-Bouhssini M, Sayyadi Z (2007) Beauveria bassiana for the control of Sunn Pest (Eurygaster integriceps) (Hemiptera: Scutelleridae) and aspects of the insect’s daily activity relevant to a mycoinsecticide. Sunn Pest Management 17:63‒79. https://doi.org/10.1080/09583150600936990
Fernandes ÉKK, Rangel DEN, Moraes ÁML, Bittencourt VREP, Roberts DW (2008) Cold activity of Beauveria and Metarhizium, and thermotolerance of Beauveria. Journal of Invertebrate Pathology 98:69‒78. https://doi.org/10.1016/j.jip.2007.10.011
Karimi J, Dara SK, Arthurs S (2019) Microbial insectcides in Iran: history, current status, challenges and perspective. Journal of invertebrate pathology 165:67‒73. https://doi.org/10.1016/j.jip.2018.02.016
Lee WW, Shin TY, Bae SM, Woo SD (2015) Screening and evaluation of entomopathogenic fungi against the green peach aphid, Myzus persicae, using multiple tools. Journal of Asia-Pacific Entomology 18(3):607‒615. https://doi.org/10.1016/j.aspen.2015.07.012
Moosavi MR, Minassian V (2021) Microbial Biopesticides: opportunities and challenges. In: Biological control of insect and mite pests in Iran (Karimi J, Madadi H, eds). Springer-Verlag, Berlin, pp 535‒550. https://doi.org/10.1007/978-3-030-63990-7_14
Parker BL, Skinner M, Costa SD, Gouli S, Reid W, El Bouhssini M (2003) Entomopathogenic fungi of Eurygaster integriceps Puton (Hemiptera: Scutelleridae): collection and characterization for development. Biological Control 27(3):260‒272. https://doi.org/10.1016/S1049-9644(03)00017-3
Parsi F (2019) Entomopathogenic fungi collected from Sunn Pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae), overwintering sites in Central Iran. Journal of Biology and Today's World 8(10):1-5. https://www.iomcworld.org/archive/jbtw-volume-8-issue-10-year-2019.html
Parsi F, Peighami Ashnaei S (2022) Screening thermotolerant isolates of Beauveria bassiana for potential management of Sunn Pest (Eurygaster integriceps Puton) in Iran. Biocontrol Science and Technology 32:1346‒1350. https://doi.org/10.1080/09583157.2022.2109595
Shin TY, Bae SM, Kim DJ, Yun HG, Woo SD (2017) Evaluation of virulence, tolerance to environmental factors and antimicrobial activities of entomopathogenic fungi against two-spotted spider mite, Tetranychus urticae. Mycoscience 58(3):204‒212. https://doi.org/10.1016/j.myc.2017.02.002
Webster R (2007) Analysis of variance, inference, multiple comparisons and sampling effects in soil research. European Journal of Soil Science 58(1):74‒82. https://doi.org/10.1111/j.1365-2389.2006.00801.x
White TJ, Bruns T, Lee SJWT, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR protocols: a guide to methods and applications (Innis MA, Gelfand DH, Sninsky J, White TJ, eds). Academic Press, San Diego, CA, pp. 315–322.http://dx.doi.org/10.1016/B978-0-12-372180-8.50042-1
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