Morphological observation and biomass formation in different edible medicinal Morchella collections (Pezizomycetes, Ascomycota)

Authors

  • Susanna Badalyan Laboratory of Fungal Biology and Biotechnology, Institute of Biology, Yerevan State University, Yerevan, Armenia
  • Narine Gharibyan Laboratory of Fungal Biology and Biotechnology, Institute of Biology, Yerevan State University, Yerevan, Armenia
  • Carmelo Gianchino Department of Life, Health and Environmental Science, University of L’Aquila, via Vetoio, 67100, Coppito, L’Aquila, Italy
  • Mirco Iotti Department of Life, Health and Environmental Science, University of L’Aquila, via Vetoio, 67100, Coppito, L’Aquila, Italy
  • Alessandra Zambonelli Dipartimento di Scienze e Tecnologie Agro-Alimentari, University of Bologna, Bologna, Italy

DOI:

https://doi.org/10.6092/issn.2531-7342/16112

Keywords:

morels, mycelia, morphology, Morchella esculenta, Morchella dunalii, Morchella importuna, Morchella disparilis, Morchella purpurascens, biomass

Abstract

The ascomycetes in the genus Morchella (commonly called morels) have a considerable economic and biotechnological value for their culinary and medicinal proprieties. However, their biotechnological interest is not limited to the fruiting body cultivation, but also in mycelial production to obtain bioactive compounds and other biotech products. In order to better exploit the biotechnological potentialities of morels it is necessary to improve the knowledge on their biology and mycelial characteristics. In this paper morphological and growth characteristics of mycelia, as well as biomass formation of Italian collections of five edible medicinal Morchella species [M. esculenta (L.) Pers., M. dunalii Boud., M. importuna M. Kuo, O'Donnell & T.J. Volk, M. disparilis Loizides & P.-A. Moreau and M. purpurascens (Krombh. ex Boud.) Jacquet.] are presented.

References

Ajmal M, Akram A, Ara A, Akhund S, Nayyar BG (2015) Morchella esculenta: an edible and health beneficial mushroom. Pakistan Journal of Food Sciences 25(2):71–78.

Arkan O, Güler P (1992) The sclerotia formations in Morchella conica Pers. Doga. Turkish Journal of Biology 16:217–226.

Badalyan SM (2012) Medicinal aspects of edible ectomycorrhizal mushrooms. In: Edible Ectomycorrhizal Mushrooms, Current Knowledge and Future Prospects (Zambonelli A, Bonito G, eds), Soil Biology, Vol. 34. Springer-Verlag, Heidelberg, pp 317–334. https://doi.org/10.1007/978-3-642-33823-6_18

Barseghyan G, Kosakyan A, Isikhuemhen O, Didukh M, Wasser S (2012) Phylogenetic analysis within genera Morchella (Ascomycota, Pezizales) and Macrolepiota (Basidiomycota, Agaricales) inferred from nrDNA ITS and EF-1alpha sequences. In: Systematics and evolution of fungi (Misra JK, Tewari JP, Deshmukh SK, eds). CRC Press, Boca Raton, pp 159–205.

Badalyan SM, Navarro-González M, Kües U (2011) Taxonomic significance of anamorphic characteristics in the life cycle of coprinoid mushrooms. In: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products, 4-7 October 2011, Arcachone, France, pp 140–154.

Badalyan SM, Zambonelli A (2019) Biotechnological exploitation of macrofungi for the production of food, pharmaceuticals and cosmeceuticals. In: Advances in Macrofungi: Diversity, Ecology and Biotechnology (Sridhar KR, Deshmukh SK, eds), First Edition. CRC Press, Boca Raton, pp 199–230. https://doi.org/10.1201/9780429504075

Beluhan S, Ranogajec A (2011) Chemical composition and non-volatile components of Croatian wild edible mushrooms. Food Chemistry 124:1076-1082. https://doi.org/10.1016/j.foodchem.2010.07.081

Bonuso E, Iotti M, Macrì A, Zambonelli A (2006) Approccio innovativo per l’identificazione molecolare dei funghi filamentosi. Micologia Italiana 35(3):33–41.

Buchalo A, Mykchaylova O, Lomberg M, Wasser SP (2009) Microstructures of vegetative mycelium of macromycetes in pure culture. Volz PA, Nevo E (eds), Alterpress, Kiev, p 208.

Bulam S, Üstün NS, Pekşen A (2018) True morel (Morchella spp.) mushroom in Turkey. Proceedings Book of International Eurasian Congress on Natural Nutrition & Healthy Life, 12-15 July 2018, Ankara, Turkey, p 7. https://www.researchgate.net/publication/330000106

Carris LM, Peever TL, McCotter SW (2015) Mitospore stages of Disciotis, Gyromitra and Morchella in the inland Pacific Northwest USA. Mycologia 107(4):729–744. https://doi.org/10.3852/14-207

Dissanayake AA, Mills GL, Bonito G, Rennick B, Nair MG (2021) Chemical composition and anti-inflammatory and antioxidant activities of extracts from cultivated morel mushrooms, species of genus Morchella (Ascomycota). International Journal of Medicinal Mushrooms 23(9):73–83. https://doi.org/10.1615/IntJMedMushrooms.2021039297

Du XH, Wu DM, He GQ, Wei W, Xu N, Li TL (2019) Six new species and two new records of Morchella in China using phylogenetic and morphological analyses. Mycologia 111:857–870. https://doi.org/10.1080/00275514.2019.1640012

Du XH, Zhao Q, Zhu L, Yang ZL (2015) A review on research advances, issues, and perspectives of morels. Mycology 6(2):78–85. https://doi.org/10.1080/21501203.2015.1016561

Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2(2):113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

Güler P, Arkan O (2000) Cultural characteristics of Morchella esculenta mycelium on some nutrients. Turkish Journal of Biology 24:783–794.

He P, Yu M, Cai Y, Liu W, Wang W, Wang S, Lie J (2019) Effect of aging on culture and cultivation of the culinary-medicinal mushrooms Morchella importuna and M. sextelata (Ascomycetes). International Journal of Medicinal Mushrooms 21(11):1089–1098. https://doi.org/10.1615/IntJMedMushrooms.2019032891

Heleno SA, Stojkovic D, Barros L, Glamoclija J, Sokovic M, Martins A, Queiroz MJR, Ferreira ICFR (2013) A comparative study of chemical composition, antioxidant and antimicrobial properties of Morchella esculenta (L.) Pers. from Portugal and Serbia. Food Research International 51:236–243. https://doi.org/10.1016/j.foodres.2012.12.020

Hibbett DS, Binder M, Bischoff JF, Blackwell M, Cannon PF, Eriksson OE, Huhndorf S, James T, Kirk PM, Lücking R Lumbsch HT, Lutzoni F, Matheny PB, McLaughlin DJ, Powell MJ, Redhead S, Schoch CL, Spatafora JW, Stalpers JA, Vilgalys R, Aime MC, Aptroot A, Bauer R, Begerow D, Benny GL, Castlebury LA, Crous PW, Dai YC, Gams W, Geiser DM, Griffith GW, Gueidan C, Hawksworth DL, Hestmark G, Hosaka K, Humber RA, Hyde KD, Ironside JE, Kõljalg U, Kurtzman CP, Larsson KH, Lichtwardt R, Longcore J, Miądlikowska J, Miller A, Moncalvo JM, Mozley-Standridge S, Oberwinkler F, Parmasto E, Reeb V, Rogers JD, Roux C, Ryvarden L, Sampaio JP, Schüßler A, Sugiyama J, Thorn RG, Tibell L, Untereiner WA, Walker C, Wang Z, Weir A, Weiss M, White MM, Winka K, Yao YJ, Zhang N (2007) A higher-level phylogenetic classification of the Fungi. Mycological Research 111(5):509–547. https://doi.org/10.1016/j.mycres.2007.03.004

Hobbs CR (1995) Medicinal mushrooms: an exploration of tradition, healing and culture. Botanica Press, Summertown, Canada, p 251.

Jander-Shagug G, Masaphy S (2010) Free radical scavenging activity of culinary-medicinal morel mushrooms, Morchella Dill. ex Pers. (Ascomycetes): relation to color and phenol contents. International Journal of Medicinal Mushrooms 12:299–307. https://doi.org/10.1615/IntJMedMushr.v12.i3.90

Fu L, Wang Y, Wang J, Yang Y, Hao L (2013) Evaluation of the antioxidant activity of extracellular polysaccharides from Morchella esculenta. Food and Function 4(6):871–879. http://doi.org/10.1039/c3fo60033e

Liu Q, Ma H, Zhang Y, Dong C (2018) Artificial cultivation of true morels: current state, issues and perspectives. Critical Reviews in Biotechnology 38(2):259–271. https://doi.org/10.1080/07388551.2017.1333082

Loizides M, Gonou-Zagou Z, Fransuas G, Drakopoulos P, Sammut C, Martinis A, Bellanger JM (2021) Extended phylogeography of the ancestral Morchella anatolica supports preglacial presence in Europe and Mediterranean origin of morels. Mycologia 113:559–573. https://doi.org/10.1080/00275514.2020.1869489

Nitha B, Fijesh PV, Janardhanan KK (2013) Hepatoprotective activity of cultured mycelium of morel mushroom, Morchella esculenta. Experimental and Toxicologic Pathology 65:105–112. https://doi.org/10.1016/j.etp.2011.06.007

Nitha B, Meera CR, Janardhanan KK (2007) Anti-inflammatory and antitumor activities of cultured mycelium of morel mushroom, Morchella esculenta. Current Science 92(2):235–239.

Ori F, Hall I, Gianchino C, Iotti M, Zambonelli A (2019) Truffles and morels: two different evolutionary strategies of fungal-plant interactions in the Pezizales. In: Plant Microbe Interface (Verma A, Tropathi S, Prasad R, eds). Springer, Cham, Switzerland, pp 69–93. https://doi.org/10.1007/978-3-030-19831-2_3

Paden JW (1972) Imperfect states and the taxonomy of the Pezizales. Persoonia 6(4):405–414. https://repository.naturalis.nl/pub/531740

Paul N, Slathia PS, Vaid A, Kumar R (2018) Traditional knowledge of Gucchi, Morchella esculenta (Ascomycetes), in Doda District, Jammu and Kashmir, India. International Journal of Medicinal Mushrooms 20(5):445–450. https://doi.org/10.1615/IntJMedMushrooms.2018025995

Pekşen A, Akdeniz H (2012) Wild mushrooms as organic product. Duzce University Faculty of Forestry Journal of Forestry 8(1):34–40.

Peng W, Chen Y, Tan H, Tang J, Gan B (2015) Artificial cultivation of morels is blooming in Sichuan. Bulletin of the world society for mushroom biology and mushroom products 13.

Peretz A, Zabari L, Pastukh N, Avital N, Masaphy S (2018) In vitro anti-leishmanial activity of a black morel, Morchella importuna (Ascomycetes). International Journal of Medicinal Mushrooms 20:71–80. https://doi.org/10.1615/IntJMedMushrooms.2018025313

Stalpers JA (1978) Identification of wood-inhabiting Aphyllophorales in pure culture. Studies in Mycology 16:1–248.

Sunil C, Xu B (2022) Mycochemical profile and health-promoting effects of morel mushroom Morchella esculenta (L.) - A review. Food Research International 159:111571. https://doi.org/10.1016/j.foodres.2022.111571

Taşkin H (2013) Detection of volatile aroma compounds of Morchella by headspace gas chromatography mass spectrometry (HS-GC/MS). Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41(1):122–125. https://doi.org/10.15835/nbha4118344

Tietel Z, Masaphy S (2017) True morels (Morchella) – nutritional and phytochemical composition, health benefits and flavor: a review. Critical Reviews in Food Science and Nutrition 58:1888–1901. https://doi.org/10.1080/10408398.2017.1285269

Tietel Z, Masaphy S (2018) Aroma-volatile profile of black morel (Morchella importuna) grown in Israel. Journal of the Science of Food and Agriculture 98:346–53. https://doi.org/10.1002/jsfa.8477

Vieira V, Fernandes A, Barros L, Glamoclija J, Ciric A, Stojkovic D, Martins A, Sokovic M, Ferreira I (2016) Wild Morchella conica Pers. from different origins: A comparative study of nutritional and bioactive properties. Journal of the Science of Food and Agriculture 96:90–98. https://doi.org/10.1002/jsfa.7063.

Volk TJ, Leonard TJ (1990) The current status of morel cultivation. Mushroom News 38(8):24–27.

Xiong C, Luo Q, Huang W, Li Q, Chen C, Chen Z, Yang Z (2017) The potential neuritogenic activity of aqueous extracts from Morchella importuna in rat pheochromocytoma cells. Food Science and Biotechnology 26:1685–1692. https://doi.org/10.1007/s10068-017-0224-x

Xiong C, Qiang L, Cheng C, Chen Z, Huang W (2016) Neuroprotective effect of crude polysaccharide isolated from the fruiting bodies of Morchella importuna against H2O2-induced PC12 cell cytotoxicity by reducing oxidative stress. Biomedicine & Pharmacotherapy 83:569–576. https://doi.org/10.1016/j.biopha.2016.07.016

Xu Y, Tang J, Wang Y, He X, Tan H, Yu Y, Chen Y, Peng W (2022) Large-scale commercial cultivation of morels: current state and perspectives. Applied Microbiology and Biotechnology 106:4401–4412. https://doi.org/10.1007/s00253-022-12012-y

Yuan BH, Li H, Liu L, Du XH (2021) Successful induction and recognition of conidiation, conidial germination and chlamydospore formation in pure culture of Morchella. Fungal Biology 125(4):285–293. https://doi.org/10.1016/j.funbio.2020.11.005

Zhao Q, Lv M, Li L, Huang W, Zhang Y, Hao Z (2021) "Temptation" and "Trap" of Morchella industry in China. Journal of Yeast and Fungal Research 19(4):232–237. https://doi.org/10.13341/j.jfr.2021.1447

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Published

2023-03-14

How to Cite

Badalyan, S., Gharibyan, N., Gianchino, C., Iotti, M., & Zambonelli, A. (2023). Morphological observation and biomass formation in different edible medicinal Morchella collections (Pezizomycetes, Ascomycota). Italian Journal of Mycology, 52(1), 50–61. https://doi.org/10.6092/issn.2531-7342/16112

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