Morphological and ecological screening of different collections of medicinal white-rot bracket fungus Ganoderma adspersum (Schulzer) Donk (Agaricomycetes, Polyporales)

Susanna M. Badalyan, Narine G. Gharibyan, Mirco Iotti, Alessandra Zambonelli

Abstract


The screening of morphological and growth characteristics of mycelial collections of medicinal bracket fungus Ganoderma adspersum (Agaricomycetes, Polyporales) of different geographical origins (Armenia, Georgia, Iran) on malt-extract agar (MEA) and potato-dextrose agar (PDA) media for 6 days, as well as in submerged culture (malt-extract, ME, 200 rt min) for 14 days at different temperature (25, 30, 35, 38 °C) was performed. Species-specific mycelial macro- and micromorphological characteristics, such as white, cottony-felt, later chamois, leathery, creamy-lemon-yellowish fast-growing colonies, round-shaped hyphal clamps, hyaline, round-shaped, smooth chlamydospore-like swellings and brownish cuticular cells, numerous tetrahedral crystals were described in agar and submerged cultures. The formation of small, dense and smooth pellets, as well as hyphal swellings during submerged growth was observed. The favorable growth temperature for studied collections of G. adspersum was 25-30 °C. The revealed cultural characteristics will be used for taxonomic identification and quality control of mycelial cultures during their biotechnological cultivation.

Keywords


Ganoderma adspersum; mycelium; morphological characteristics; growth rate; chlamydospores; cuticular cells; pellets

Full Text:

PDF (English)

References


Adaskaveg JE, Gilbertson RL (1986) Cultural studies and genetics of sexuality of Ganoderma lucidum and G. tsugae in relation to the taxonomy of the G. lucidum complex. Mycologia 78:694–705. https://doi.org/10.2307/3807513

Adaskaveg JE, Gilbertson RL (1989) Cultural studies of four North American species in the Ganoderma lucidum complex with comparisons to G. lucidum and G. tsugae. Mycological Research 92:182–191. https://doi.org/10.1016/S0953-7562(89)80010-3

Badalyan SM, Sakeyan CZ (2004) Morphological, physiological and growth characteristics of mycelium of several medicinal mushrooms (Aphyllophoromycetideae). International Journal of Medicinal Mushrooms 6:347-360. https://doi.org/10.1615/IntJMedMushr.v6.i4.50

Badalyan SM, Gharibyan NG (2015) Proteolytic and milk-coagulating activities of mycelia of several polypore mushrooms. In: Current Mycology in Russia, Proceedings of the 3rd International Mycological Forum, vol. 5 (Dyakov YT, Sergeev YV, eds), 14-15 April 2015, Moscow, pp 275-277, [in Russian].

Badalyan SM, Kües U (2015) Morphological characteristics of vegetative mycelia and anamorphs in different collections of xylotrophic basidiomycetous mushrooms. In: Biodiversity and ecology of fungi and fungiform organisms of the Northern Eurasia, Proceedings of All-Russian conf. with international participation (Mukhin et al. eds), 20-24 April 2015, Yekaterinburg, pp 300-302.

Badalyan SM, Gharibyan NG (2016) Diversity of polypore bracket mushrooms, Polyporales (Agaricomycetes) recorded in Armenia and their medicinal properties. International Journal of Medicinal Mushrooms 18:347-354. https://doi.org/10.1615/IntJMedMushrooms.v18.i4.80

Badalyan SM, Gharibyan NG (2017a) Characteristics of mycelial structures of different fungal collections. YSU Press, Yerevan.

Badalyan SM, Gharibyan NG (2017b) Antiphytopathogenic activity of mycelia polypore mushrooms (Аgaricomycetes, Polyporales). In: Current Mycology in Russia, Proceedings of the 4th Congress Russian Mycologists, vol. 7 (Dyakov YT, Sergeev YV, eds), 12-14 April 2017. Moscow, pp 207-208, [in Russian].

Badalyan SM, Zambonelli A (2019) Biotechnological exploitation of macrofungi for the production of food, pharmaceuticals and cosmeceuticals. In: Advances in macrofungi: diversity, ecology and biotechnology (Sridhar KR, Deshmukh SK, eds). CRC Press, Boca Raton, pp 199-230.

Badalyan SM, Gharibyan NG, Asatryan AN (2012a) Morphological and ecological characteristics of mycelia of several Ganoderma P. Karst. species. In: Current Mycology in Russia, Proceedings of the 3rd Congress Russian Mycologists, vol. 3 (Dyakov YT, Sergeev YV, eds), 10-12 October 2012, Moscow, pp 141-142, [in Russian].

Badalyan SM, Gharibyan NG, Iotti M, Zambonelli A (2012b) Morphological and genetic characteristics of different collections of Ganoderma P. Karst. species. In: Mushroom Science XVIII, Proceedings of the 18th ISMS Congress (Zhang J, Wang H, Chen M, eds), 26-30 August 2012, Beijing, China Agriculture press, pp 247-254.

Badalyan SM, Shnyreva AV, Iotti M, Zambonelli A (2015) Genetic resources and mycelial characteristics of several medicinal polypore mushrooms (Polyporales, Basidiomycetes). International Journal of Medicinal Mushrooms 17(4):371-384. https://doi.org/10.1615/IntJMedMushrooms.v17.i4.60

Badalyan SM, Barkhudaryan A, Rapior S (2019) Recent progress in research on the pharmacological potential of mushrooms and prospects for their clinical application. In: Medicinal Mushrooms: Recent Advances in Research and Development (Agrawal D, Krishnan M, eds). Springer-Verlag (in press).

Boh B, Berovic M, Wraber B, Hodzar D, Habijanic J, Zore I (2004) Ganoderma lucidum (W. Curt.: Fr.) Lioyd and G. applanatum (Pers.) Pat. (Aphyllophoromycetideae) from Slovenian habitats: cultivation, isolation, and testing of active compounds. International Journal of Medicinal Mushrooms 6(1):15−32. https://doi.org/10.1615/IntJMedMushr.v6.i1.20

Bukhalo AS (1988) Higher edible basidiomycetes in pure culture. Nauk. Dumka, Kiev.

Bukhalo A, Mykchaylova O, Lomberg M, Wasser SP, Volz PA, Nevo E (2009) Microstructures of vegetative mycelium of macromycetes in pure culture. Alterpress, Kiev.

Clémençon H (2012) Cytology and plectology of the hymenomycetes. 2nd revised edn. J. Cramer in der Gebr. Borntraeger Verlagsbuchhandlung, Stuttgart.

Gao Y, Zhou S, Huang M, Xu A (2003) Antibacterial and antiviral value of the genus Ganoderma P. Karst. species (Aphyllophoromycetideae): a review. International Journal of Medicinal Mushrooms 5(3):235-246. https://doi.org/10.1615/InterJMedicMush.v5.i3.20

Gargano ML, van Griensven LJLD, Isikhuemhen OS, Venturella G, Wasser SP, Zervakis GI (2017) Medicinal mushrooms: valuable biological resources of high exploitation potential. Plant Biosystem 151(3):548–565. https://doi.org/10.1080/11263504.2017.1301590

Hong SG, Jung HS (2004) Phylogenetic analysis of Ganoderma based on nearly complete mitochondrial small-subunit ribosomal DNA sequences. Mycologia 96(4):742-755. https://doi.org/10.1080/15572536.2005.11832922

Hseu R (1990) An identification system for cultures of Ganoderma species. PhD dissertation, National Taiwan University, Taipei.

Jargalmaa S, Eimes JA, Park MS, Park JY, Oh SY, Lim YW (2017) Taxonomic evaluation of selected Ganoderma species and database sequence validation. PeerJ 5:e3596. https://doi.org/10.7717/peerj.3596

Jayasinghe C, Imtiaj A, Hur H, Lee GW, Lee TS, Lee UY (2008) Favorable culture conditions for mycelial growth of Korean wild strains in Ganoderma lucidum. Mycrobiology 36(1):28-33. https://doi.org/10.4489/MYCO.2008.36.1.028

Jeong YT, Jeong SC, Yang BK, Islam R, Song CH (2009) Optimal culture conditions for mycelial growth and exo-polymer production of Ganoderma applanatum. Microbiology 37(2):89-93. https://doi.org/10.4489/MYCO.2009.37.2.089

Jo WS, Cho YJ, Cho DH, Park SD, Yoo YB, Seok SJ (2009) Culture conditions for the mycelial growth of Ganoderma applanatum. Mycobiology 37(2):94-102. https://doi.org/10.4489/MYCO.2009.37.2.094

Kües U, Badalyan SM (2017) Making use of genomic information to explore the biotechnological potential of medicinal mushrooms. In: Medicinal Plants and Fungi: Recent Advances in Research and Development, vol. 2 (Agrawal D, Tsay HS, Shyur LF, Wu YC, Wang SY, eds), Medicinal and Aromatic Plants of the World book series, vol. 4. Springer Nature, Singapore, pp 397-458. https://doi.org/10.1007/978-981-10-5978-0_13

Kües U, Badalyan SM, Gießler A, Dörnte B (2016) Asexual sporulation in Agaricomycetes. In: The Mycota: Growth, Differentiation and Sexuality, vol. I, 3rd edn. (Wendland J, ed.). Springer International Publishing, Cham, pp. 269-328. https://doi.org/10.1007/978-3-319-25844-7_12

Luangharn T, Karunarathna SC, Khan S, Xu JC, Mortimer PE, Hyde KD (2017) Antibacterial activity, optimal culture conditions and cultivation of the medicinal Ganoderma australe, new to Thailand. Mycosphere 8(8):1108–1123. https://doi.org/10.5943/mycosphere/8/8/11

Mohanty PS, Harsh NSK, Pandey A (2011) First report of Ganoderma resinaceum and G. weberianum from north India based on ITS sequence analysis and micromorphology. Mycosphere 2(4):469–474.

Moncalvo JM (2000) Systematics of Ganoderma. In: Ganoderma diseases of perennial crops (Flood J, Bridge PD, Holderness M, eds). CABI Bioscience; Egham, pp. 23–45.

Moncalvo JM (2005) Molecular systematics of Ganoderma: What is Reishi? International Journal of Medicinal Mushrooms 7(3):353–354. https://doi.org/10.1615/IntJMedMushrooms.v7.i3.160

Moncalvo J, Wang H, Hseu R (1995) Gene phylogeny of the Ganoderma lucidum complex based on ribosomal DNA sequences. Comparison with traditional taxonomic characters. Mycological Research 99(12):1489-1499. https://doi.org/10.1016/S0953-7562(09)80798-3

Nobles MK (1965) Identification of cultures of wood-inhabiting Hymenomycetes. Canadian Journal of Botany 43:1097-1139.

Paterson RRM (2006) Ganoderma – a therapeutic fungal biofactory. Phytochemistry 67(18):1985-2001. https://doi.org/10.1016/j.phytochem.2006.07.004

Phillips R (1981) Mushrooms and other fungi of Great Britain and Europe. Pan Books, London.

Ryvarden L (1991) Genera of polypores. Nomenclature and taxonomy. Fungiflora, Oslo.

Ryvarden L, Gilbertson RL (1993) European polypores. Part 1. Abortiporus-Lindtneria. Fungiflora, Oslo.

Saltarelli R, Ceccaroli P, Buffalini M, Vallorani L, Casadei L, Zambonelli A, Iotti M, Badalyan S, Stocchi V (2015) Biochemical characterization, antioxidant and antiproliferative activities of different Ganoderma collections. Journal of Molecular Microbiology and Biotechnology 25(1):16-25. https://doi.org/10.1159/000369212

Singh S, Harsh NSK, Suresh Chandra S (2016) Diversity of Ganoderma lucidum collected from different host tree species from north India. The Indian Forester 142(12).

Stalpers JH (1978) Identification of wood inhabiting Aphyllophorales in pure culture. Studies in Mycology 16:1-248.

Steyaert RL (1980) Study of some Ganoderma species. Bulletin du Jardin Botanique National de Belgique 150:135-186.

Szedlay GY, Jakucs E, Bóka K, Boldizsár I (1996) Macro- and micromorphological characteristics of Ganoderma lucidum Karsten strains isolated in Hungary. Annales Historico-Naturales Musei Nationalis Hungarici 88:57-68.

Taofiq O, González-Paramás AM, Martins A, Barreiro MF, Ferreira ICFR (2016) Mushrooms extracts and compounds in cosmetics, cosmeceuticals and nutricosmetics - A review. Industrial Crops and Products 90:38–48. https://doi.org/10.1016/j.indcrop.2016.06.012

Taofiq O, Heleno SA, Calhelha RC, Alves, MJ, Barros, L, Gonzalez-Paramas, AM, Barreiro, MF, Ferreira, ICFR (2017) The potential of Ganoderma lucidum extracts as bioactive ingredients in topical formulations, beyond its nutritional benefits. Food and Chemical Toxicology 108:139-147. https://doi.org/10.1016/j.fct.2017.07.051

Teplyakova TV, Kosogova TA (2016) Antiviral effect of Agaricomycetes mushrooms. International Journal of Medicinal Mushrooms 18(5):375–386. https://doi.org/10.1615/IntJMedMushrooms.v18.i5.10

Tsivileva O, Nguyen T, Long VU, Yurasov N, Chernyshova M, Petrov A, Galushka V, Markin A, Koftin O (2016) Vietnamese Ganoderma: growth, peculiarities, and low-molecular composition compared to European and Siberian strains. Turkish Journal of Botany 40:269-286. https://doi.org/10.3906/bot-1410-15

Wang B, Hua JA (1991) Cultural atlas of some Ganoderma cultures. Food Industry Research and Development Institute, Hsinchu.




DOI: 10.6092/issn.2531-7342/9092

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Susanna M. Badalyan, Narine G. Gharibyan, Mirco Iotti, Alessandra Zambonelli

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.