Schizophyllum amplum (Agaricales, Schizophyllaceae): A rare Basidiomycete from Malta and Estonia

Carmel Sammut, Pablo Alvarado, Irja Saar

Abstract


Schizophyllum amplum (Agaricales, Schizophyllaceae) is reported for the first time in Malta and Estonia. The collections from Malta were examined morphologically and compared with previously published accounts of this species. The specimens from Estonia and Malta were sequence d after PCR amplification with ITS1F and ITS4 for ITS region. The sequences were then compared to other sequences deposited in public databases using BLASTn. Genetically the ITS rDNA data obtained from the Maltese and Estonian specimens are 99% similar but show some differences from other sequences of S. amplum (L43381 and L43382) deposited in GenBank. A multigenetic approach with a larger number of specimens should be employed to define the currently accepted genetic limits for Schizophyllum species.

Keywords


Auriculariopsis ampla; ITS rDNA; mycobiota; Mediterranean; morphology; phylogeny

Full Text:

PDF (English)

References


Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403–410. https://doi.org/10.1016/S0022-2836(05)80360-2

Cooke WB (1961) The genus Schizophyllum. Mycologia 53:575–99. https://doi.org/10.2307/3756459

Erikkson J, Ryvarden L (1975) The Corticiaceae of North Europe, 3: 287–546, Fungiflora, Oslo, Norway.

Fries EM (1815) Observationes Mycologicae. sumptibus G. Bonnieri.

Gardes M, T.D. Bruns TD (1993) ITS primers with enhanced specificity for Basidiomycetes—application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

Kõljalg U, et al. (2013) Towards a unified paradigm for sequence-based identification of Fungi. Molecular Ecology 22(21): 5271–5277. https://doi.org/10.1111/mec.12481

Léveillé M J-H (1848) Fragments Mycologiques. Annales des Sciences Naturelles, série 3, 9: 119–144.

Maire R (1902) Recherches cytologiques & taxonomiques sur les Basidiomycètes. Bulletin de la Société Mycologique de France, 18 (suppl.): 1–209.

Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8(19):4321–4325. https://doi.org/10.1093/nar/8.19.4321

Nakasone KK (1996) Morphological and molecular studies on Auriculariopsis albomellea and Phlebia albida and a reassessment of A. ampla, Mycologia 88(5): 762–775. https://doi.org/10.2307/3760971

Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Uppsala, Evolutionary Biology Centre, Uppsala University.

Ohm RA, et al. (2010) Genome sequence of the model mushroom Schizophyllum commune. Nature Biotechnology 28(9): 957–965. https://doi.org/10.1038/nbt.1643

Robinson K, Burnham A, Kibby G, Ainsworth M (2011) Fungi associated with poplars. Field Mycology 12(2): 56–67. https://doi.org/10.1016/j.fldmyc.2011.03.007

Robledo GL, Popoff OF, Amarilla LD, Moncalvo J-M, Urcelay C (2014) Schizophyllum leprieurii and the Schizophyllum umbrinum lineage (Agaricales, Basidiomycota) in Argentina. Lilloa 51(1): 87–96.

Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.

Siqueira JPZ, Sutton D, Gené J, García D, Guevara-Suarez M, Decock C, Wiederhold N, Guarro J (2016) Schizophyllum radiatum, an emerging fungus from human respiratory tract. Journal of Clinical Microbiology 54(10): 2491–2497. https://doi.org/10.1128/JCM.01170-16

Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690. https://doi.org/10.1093/bioinformatics/btl446

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28(10): 2731–2739. https://doi.org/10.1093/molbev/msr121

White TJ, Bruns TD, RUNS, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky J, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego.

Wojewoda W (2006) Auriculariopsis albomellea (Agaricales, Schizophyllaceae) new for Poland, Acta Mycologica 41(1): 49–54. https://doi.org/10.5586/am.2006.008




DOI: 10.6092/issn.2531-7342/9426

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Carmel Sammut, Pablo Alvarado, Irja Saar

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.